Question 1

The DNA content of a patient's tumor cells is reported as 2.0,and the DNA content of diploid cells is 1.0.What is the DNA index?

Accepted Answer

A)  2.0
B)  3.0
C)  1.0
D)  0.5E) All of the above
F) B) and C)

Question 2

A single group of cells possessing identical aberrant phenotypes is defined as:

Accepted Answer

A)  Monoclonal
B)  Clonal
C)  Tumorigenic
D)  Contour gatedE) None of the above
F) A) and D)

Question 3

Explain why flow cytometry is of limited use in identifying AML.

Accepted Answer

The WHO classification of AML employs cy...

Question 4

A blood sample from a patient suspected of having ALL is received in the flow lab for immunophenotyping.The analysis is performed,and standard B-cell markers are present,but the patient is also CD33-positive.What is the most likely reason for this occurrence?

Accepted Answer

A)  The patient has a poorer prognosis than standard ALL.
B)  The patient is responding to the chemotherapy.
C)  The patient has ALL with aberrant myeloid cellular antigens.
D)  It cannot be determined by the information given.E) A) and B)
F) None of the above

Question 5

What is one advantage to using monoclonal antibodies rather than polyclonal antibodies in immunophenotyping?

Accepted Answer

A)  They are commercially available.
B)  The antigen can be recognized even if some parts of it are abnormal.
C)  They have high purity and reproducibility.
D)  They can be directly attached to fluorochromes.E) A) and C)
F) B) and C) C

Question 6

Which of the following is(are) the clinical application(s) of flow cytometry?

Accepted Answer

A)  Assessment of total body iron
B)  Immunophenotyping
C)  DNA amplification
D)  Cytogenetic analysisE) B) and D)
F) A) and B)

Question 7

Name at least two pitfalls in immunophenotyping mature lymphoid malignancies,and explain how you would correct this.

Accepted Answer

Some malignant B- cell lymphoproliferati...

Question 8

A flow instrument printout reveals a single scatter population of small size and low complexity.Ninety percent of the cells in that population have CD2 on their surface.What is the most probable lineage of the unknown population?

Accepted Answer

A)  NK cells
B)  T cells
C)  B cells
D)  MonocytesE) All of the above
F) A) and C)

Question 9

CD34 enumeration is useful in bone marrow and peripheral blood stem cell transplantation.For this enumeration,the EWGCCA recommendations include proper technique and interpretation of the flow cytometry analysis.Which of the following standards was recommended for proper acquisition of events?

Accepted Answer

A)  Include platelets and unlysed red blood cells and debris
B)  Include only CD34 dim staining populations
C)  Use bright fluorochrome conjugates of class II or III monoclonal antibodies that detect all glycoforms of CD34
D)  Acquire only 10 CD34 positive cellsE) A) and B)
F) C) and D)

Question 10

Cells can be separated during data analysis by gating.This process of isolating cells can be based on what properties?

Accepted Answer

A)  Cell size and granularity
B)  Cell density
C)  Shape of the nucleus
D)  Color of the cytoplasmE) B) and C)
F) A) and D)

Question 11

In diagnosing PNH,which of the following assays is most sensitive to its detection?

Accepted Answer

A)  GPI anchor phenotype
B)  Ham test
C)  Sucrose lysis test
D)  Osmotic fragility testE) A) and D)
F) None of the above

Question 12

Which markers are useful in immunophenotyping for leukemia/lymphoma of T cell-lineage?

Accepted Answer

A)  CD103,CD61,and CD59
B)  CD38,CD59,and CD13
C)  CD2,CD3,CD4,CD5,CD7,and CD8
D)  CD55,CD25,and kappaE) C) and D)
F) B) and D)

Question 13

Neoplasms are made up of a population of identical cells or a clone of cells.When identified,clone can lead to the discovery of a lymphoid malignancy or other lymphoproliferative disorder.Clonality be detected by:

Accepted Answer

A)  The uniform expression of one immunoglobulin light chain
B)  A mixture of cells with either kappa or lambda light chains
C)  A surface immunoglobulin detection
D)  The bright intensity of all monoclonal markersE) A) and B)
F) C) and D)

Question 14

Which analyte is the best indicator of the overall picture of the immune system in an HIV-positive patient?

Accepted Answer

A)  Absolute CD4 count
B)  Relative lymphocyte count
C)  WBC count
D)  Relative CD4 countE) A) and D)
F) A) and B) A

Question 15

Choose the correct statement regarding the IRF from the following choices:

Accepted Answer

A)  It is directly proportional to the degree of bone marrow turnover.
B)  It is indirectly proportional to the average age of the reticulocytes.
C)  It cannot be used to monitor erythropoietin therapy.
D)  It cannot be used to monitor bone marrow recovery after transplant.E) All of the above
F) None of the above

Question 16

Which of the following is not the component(s) of a flow cytometer?

Accepted Answer

A)  Photomultiplier tubes
B)  Sheath fluid
C)  Flow chamber
D)  Hemoglobin chamberE) B) and C)
F) A) and D)

Question 17

An unknown cell resembling a hematogone has CD19 and CD20 antigens.What is the unknown cell's lineage?

Accepted Answer

A)  Myeloid
B)  Monocytic
C)  Lymphoid
D)  ErythroidE) None of the above
F) B) and D)

Question 18

The expression of several antigens from two different lineages complicates the categorizing the diagnosis as AML or ALL.This biphenotypic expression toward more than one lineage restricts the use of immunophenotyping.In differentiating AML and ALL,staining with which of the following cytochemical stains indicates AML?

Accepted Answer

A)  PAS
B)  Giemsa
C)  Myeloperoxidase
D)  Thiazole orangeE) A) and B)
F) B) and C)

Question 19

The percentage of CD4 positive lymphocytes is determined by flow cytometry using staining with fluorescent-tagged antibodies against CD3 and CD4 followed by whole blood RBC lysis.What calculations does the dual platform use to determine the absolute number of CD4 positive cells?

Accepted Answer

A)  Absolute CD4 count × 100
B)  Number of CD4+ cells × 100
C)  Absolute lymphocyte count × % CD4+ lymphocytes/100
D)  % lymphocytes × % CD4+ lymphocytes/100E) None of the above
F) C) and D) C

Question 20

An HIV-positive patient has his blood drawn to get his CD4 count.His results are as follows: WBC count: 2.0 × 10⁹/L with 15% lymphocytes.CD4 positive cells are 10% of the total lymphocyte count.What is this patient's absolute CD4 count,and what does this mean?

Accepted Answer

A)  The patient's absolute CD4 count is 300/mcL,and he has AIDS.
B)  The patient's absolute CD4 count is 180/mcL,and he has AIDS.
C)  The patient's absolute CD4 count is 300/mcL,and he does not have AIDS.
D)  The patient's absolute CD4 count is 120/mcL,and he does have AIDS.E) B) and D)
F) A) and B)

Exam 40: Flow Cytometry

The DNA content of a patient's tumor cells is reported as 2.0,and the DNA content of diploid cells is 1.0.What is the DNA index?

Correct Answer
verified

A single group of cells possessing identical aberrant phenotypes is defined as:

Correct Answer
verified

Explain why flow cytometry is of limited use in identifying AML.

Correct Answer
verified
The WHO classification of AML employs cy...
View Answer

A blood sample from a patient suspected of having ALL is received in the flow lab for immunophenotyping.The analysis is performed,and standard B-cell markers are present,but the patient is also CD33-positive.What is the most likely reason for this occurrence?

Correct Answer
verified

What is one advantage to using monoclonal antibodies rather than polyclonal antibodies in immunophenotyping?

Correct Answer
verified
C

Which of the following is(are) the clinical application(s) of flow cytometry?

Correct Answer
verified

Name at least two pitfalls in immunophenotyping mature lymphoid malignancies,and explain how you would correct this.

Correct Answer
verified
Some malignant B- cell lymphoproliferati...
View Answer

A flow instrument printout reveals a single scatter population of small size and low complexity.Ninety percent of the cells in that population have CD2 on their surface.What is the most probable lineage of the unknown population?

Correct Answer
verified

CD34 enumeration is useful in bone marrow and peripheral blood stem cell transplantation.For this enumeration,the EWGCCA recommendations include proper technique and interpretation of the flow cytometry analysis.Which of the following standards was recommended for proper acquisition of events?

Correct Answer
verified

Cells can be separated during data analysis by gating.This process of isolating cells can be based on what properties?

Correct Answer
verified

In diagnosing PNH,which of the following assays is most sensitive to its detection?

Correct Answer
verified

Which markers are useful in immunophenotyping for leukemia/lymphoma of T cell-lineage?

Correct Answer
verified

Neoplasms are made up of a population of identical cells or a clone of cells.When identified,clone can lead to the discovery of a lymphoid malignancy or other lymphoproliferative disorder.Clonality be detected by:

Correct Answer
verified

Which analyte is the best indicator of the overall picture of the immune system in an HIV-positive patient?

Correct Answer
verified
A

Choose the correct statement regarding the IRF from the following choices:

Correct Answer
verified

Which of the following is not the component(s) of a flow cytometer?

Correct Answer
verified

An unknown cell resembling a hematogone has CD19 and CD20 antigens.What is the unknown cell's lineage?

Correct Answer
verified

Correct Answer
verified

The percentage of CD4 positive lymphocytes is determined by flow cytometry using staining with fluorescent-tagged antibodies against CD3 and CD4 followed by whole blood RBC lysis.What calculations does the dual platform use to determine the absolute number of CD4 positive cells?

Correct Answer
verified
C

An HIV-positive patient has his blood drawn to get his CD4 count.His results are as follows: WBC count: 2.0 × 10⁹/L with 15% lymphocytes.CD4 positive cells are 10% of the total lymphocyte count.What is this patient's absolute CD4 count,and what does this mean?

Correct Answer
verified

Exam 40: Flow Cytometry

The DNA content of a patient's tumor cells is reported as 2.0,and the DNA content of diploid cells is 1.0.What is the DNA index?

Correct AnswerverifiedShow Answer

A single group of cells possessing identical aberrant phenotypes is defined as:

Correct AnswerverifiedShow Answer

Explain why flow cytometry is of limited use in identifying AML.

Correct AnswerverifiedThe WHO classification of AML employs cy...View AnswerShow Answer

A blood sample from a patient suspected of having ALL is received in the flow lab for immunophenotyping.The analysis is performed,and standard B-cell markers are present,but the patient is also CD33-positive.What is the most likely reason for this occurrence?

Correct AnswerverifiedShow Answer

What is one advantage to using monoclonal antibodies rather than polyclonal antibodies in immunophenotyping?

Correct AnswerverifiedC

Which of the following is(are) the clinical application(s) of flow cytometry?

Correct AnswerverifiedShow Answer

Name at least two pitfalls in immunophenotyping mature lymphoid malignancies,and explain how you would correct this.

Correct AnswerverifiedSome malignant B- cell lymphoproliferati...View AnswerShow Answer

A flow instrument printout reveals a single scatter population of small size and low complexity.Ninety percent of the cells in that population have CD2 on their surface.What is the most probable lineage of the unknown population?

Correct AnswerverifiedShow Answer

CD34 enumeration is useful in bone marrow and peripheral blood stem cell transplantation.For this enumeration,the EWGCCA recommendations include proper technique and interpretation of the flow cytometry analysis.Which of the following standards was recommended for proper acquisition of events?

Correct AnswerverifiedShow Answer

Cells can be separated during data analysis by gating.This process of isolating cells can be based on what properties?

Correct AnswerverifiedShow Answer

In diagnosing PNH,which of the following assays is most sensitive to its detection?

Correct AnswerverifiedShow Answer

Which markers are useful in immunophenotyping for leukemia/lymphoma of T cell-lineage?

Correct AnswerverifiedShow Answer

Neoplasms are made up of a population of identical cells or a clone of cells.When identified,clone can lead to the discovery of a lymphoid malignancy or other lymphoproliferative disorder.Clonality be detected by:

Correct AnswerverifiedShow Answer

Which analyte is the best indicator of the overall picture of the immune system in an HIV-positive patient?

Correct AnswerverifiedA

Choose the correct statement regarding the IRF from the following choices:

Correct AnswerverifiedShow Answer

Which of the following is not the component(s) of a flow cytometer?

Correct AnswerverifiedShow Answer

An unknown cell resembling a hematogone has CD19 and CD20 antigens.What is the unknown cell's lineage?

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

The percentage of CD4 positive lymphocytes is determined by flow cytometry using staining with fluorescent-tagged antibodies against CD3 and CD4 followed by whole blood RBC lysis.What calculations does the dual platform use to determine the absolute number of CD4 positive cells?

Correct AnswerverifiedC

An HIV-positive patient has his blood drawn to get his CD4 count.His results are as follows: WBC count: 2.0 × 10⁹/L with 15% lymphocytes.CD4 positive cells are 10% of the total lymphocyte count.What is this patient's absolute CD4 count,and what does this mean?

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
The WHO classification of AML employs cy...
View Answer

Correct Answer
verified

Correct Answer
verified
C

Correct Answer
verified

Correct Answer
verified
Some malignant B- cell lymphoproliferati...
View Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
A

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
C

Correct Answer
verified