Question 1

A subunit vaccine is prepared by extensive manipulation of the genome of the pathogen.

Accepted Answer

A) True 
 B)False

Question 2

Mutagens are useful in biotechnology research for

Accepted Answer

A)   producing organisms with altered phenotypes.
B)   producing new organisms which have beneficial traits from two or more organisms.
C)   producing DNA fragments for cloning.
D)   removing undesirable traits from microbes.
E)   selecting genetic mutants resistant to radioactivity.F) A) and D)
G) B) and E)

Question 3

Discuss one of the pros and cons regarding the application of recombinant DNA technology.

Accepted Answer

Topics can include: the improvement of c...

Question 4

A microarray is assembled using

Accepted Answer

A)   single-stranded DNA and silicon chips.
B)   restriction enzymes.
C)   agarose and nitrocellulose.
D)   gold beads and magnets.
E)   reverse transcriptase and DNA polymerase.F) A) and B)
G) None of the above

Question 5

A northern blot differs from a Southern blot in the

Accepted Answer

A)   size of the genetic sequences involved.
B)   presence or absence of a nitrocellulose membrane.
C)   type of nucleic acid being isolated.
D)   number of genetic sequences detected.
E)   type of probe used.F) A) and B)
G) B) and C)

Question 6

Synthetic nucleic acids are useful as

Accepted Answer

A)   primers for PCR.
B)   DNA probes.
C)   antisense RNAs.
D)   DNA probes and antisense RNAs.
E)   DNA probes, primers, and antisense RNAs.F) All of the above
G) D) and E)

Question 7

Subunit vaccines are safer than traditional vaccines because they

Accepted Answer

A)   do not pose a risk for causing the disease.
B)   are acellular.
C)   are administered in food.
D)   are acellular and do not pose a risk for causing the disease.
E)   are acellular and can be administered in food.F) A) and B)
G) A) and C)

Question 8

Probes used for detecting genetic sequences are frequently composed of

Accepted Answer

A)   gold beads coated with DNA.
B)   silicon chips.
C)   synthetic nucleic acids and labeled conjugates, such as fluorescent dyes.
D)   restriction enzymes.
E)   plasmids with a marker sequence.F) B) and D)
G) A) and D)

Question 9

If all the following DNA fragments were analyzed on an electrophoresis gel, which one would migrate farthest from the negative electrode?

Accepted Answer

A)   5000 base pairs
B)   250 base pairs
C)   750 base pairs
D)   1000 base pairs
E)   2500 base pairsF) A) and E)
G) C) and D)

Question 10

If you started with a single DNA molecule, how many would you have at the end of six PCR cycles?

Accepted Answer

A)   6
B)   16
C)   32
D)   64
E)   100F) All of the above
G) A) and C)

Question 11

Some of the strongest opposition to the application of recombinant DNA technology concerns

Accepted Answer

A)   correcting gene defects in animals.
B)   pest control measures.
C)   sequencing of the human genome.
D)   DNA fingerprinting.
E)   modification of food crops.F) A) and D)
G) B) and C)

Question 12

What are the ʺsticky endsʺ produced by some restriction enzymes?

Accepted Answer

A)   Sticky ends are DNA strands with an extra hydroxyl group on the end.
B)   Sticky ends are able to hydrogen bond with complementary strands.
C)   Sticky ends are short single-strand ends of a DNA molecule.
D)   Sticky ends are short single-strand ends of DNA able to hydrogen bond with complementary strands.
E)   Sticky ends are DNA strands able to form phosphate bonds with free DNA ends.F) A) and E)
G) D) and E)

Question 13

Examples of recent accomplishments in the use of recombinant DNA technology include

Accepted Answer

A)   a cure for HIV.
B)   production of gene modified human embryos.
C)   production of new emerging disease agents.
D)   production of a peach-apple hybrid plant.
E)   gene therapy to correct an immune system deficiency in humans.F) C) and D)
G) A) and C)

Question 14

If a researcher used Escherichia coli DNA polymerase instead of Thermus aquaticus DNA polymerase in the PCR procedure, what would be the result?

Accepted Answer

A)   Many mistakes would occur.
B)   DNA replication would occur twice as fast as normal.
C)   DNA replication would occur more slowly than normal.
D)   DNA replication would not occur at all.
E)   DNA replication would stop after one cycle.F) A) and B)
G) B) and E)

Question 15

An ancient application of biotechnology is

Accepted Answer

A)   production of cheese.
B)   both traditional agriculture and the selective breeding of plants.
C)   the practice of medicine.
D)   selective breeding of plants.
E)   traditional agriculture.F) A) and E)
G) A) and C)

Question 16

One of two men may be the father of a child. This figure shows the results of a DNA fingerprint analysis to determine paternity. M is the mother, C is the child, A and B are the two men. What is the best interpretation of these results?

Accepted Answer

A)   Neither man is this childʹs father.
B)   The man identified as A is probably the father.
C)   The man identified as B is probably the father.
D)   Either man could be this childʹs father.
E)   Paternity cannot be determined from this data.F) A) and B)
G) All of the above

Question 17

Genetic (screening/sequencing/cloning) can be used to detect mutant genes associated with genetic diseases in individuals before any clinical symptoms are noted.

Accepted Answer

The answer of Genetic (screening/sequencing/cloning) can be used to detect...

Question 18

The northern blot is a technique used to detect specific RNA molecules in a larger population of molecules that have been separated by gel (electroporation/electrophoresis).

Accepted Answer

The answer of The northern blot is a technique used...

Question 19

Which of the following recombinant tools is NOT used in DNA fingerprinting?

Accepted Answer

A)   gel electrophoresis
B)   reverse transcription
C)   restriction enzyme digestion
D)   PCR
E)   Neither PCR nor gel electrophoresis is used.F) A) and B)
G) None of the above

Question 20

Small RNAs that bind to an mRNA and alter its expression are known as (antisense/probes/restriction) RNAs.

Accepted Answer

The answer of Small RNAs that bind to an mRNA...

Exam 8: Recombinant Dna Technology

A subunit vaccine is prepared by extensive manipulation of the genome of the pathogen.

Correct AnswerverifiedShow Answer

If a researcher used Escherichia coli DNA polymerase instead of Thermus aquaticus DNA polymerase in the PCR procedure, what would be the result?

Correct AnswerverifiedShow Answer

Probes used for detecting genetic sequences are frequently composed of

Correct AnswerverifiedShow Answer

A microarray is assembled using

Correct AnswerverifiedShow Answer

Mutagens are useful in biotechnology research for

Correct AnswerverifiedShow Answer

If all the following DNA fragments were analyzed on an electrophoresis gel, which one would migrate farthest from the negative electrode?

Correct AnswerverifiedShow Answer

An ancient application of biotechnology is

Correct AnswerverifiedShow Answer

One of two men may be the father of a child. This figure shows the results of a DNA fingerprint analysis to determine paternity. M is the mother, C is the child, A and B are the two men. What is the best interpretation of these results?

Correct AnswerverifiedShow Answer

The northern blot is a technique used to detect specific RNA molecules in a larger population of molecules that have been separated by gel (electroporation/electrophoresis).

Correct AnswerverifiedShow Answer

If you started with a single DNA molecule, how many would you have at the end of six PCR cycles?

Correct AnswerverifiedShow Answer

Subunit vaccines are safer than traditional vaccines because they

Correct AnswerverifiedShow Answer

Some of the strongest opposition to the application of recombinant DNA technology concerns

Correct AnswerverifiedShow Answer

Small RNAs that bind to an mRNA and alter its expression are known as (antisense/probes/restriction) RNAs.

Correct AnswerverifiedShow Answer

Discuss one of the pros and cons regarding the application of recombinant DNA technology.

Correct AnswerverifiedTopics can include: the improvement of c...View AnswerShow Answer

Synthetic nucleic acids are useful as

Correct AnswerverifiedShow Answer

What are the ʺsticky endsʺ produced by some restriction enzymes?

Correct AnswerverifiedShow Answer

Genetic (screening/sequencing/cloning) can be used to detect mutant genes associated with genetic diseases in individuals before any clinical symptoms are noted.

Correct AnswerverifiedShow Answer

Examples of recent accomplishments in the use of recombinant DNA technology include

Correct AnswerverifiedShow Answer

Which of the following recombinant tools is NOT used in DNA fingerprinting?

Correct AnswerverifiedShow Answer

A northern blot differs from a Southern blot in the

Correct AnswerverifiedShow Answer

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Topics can include: the improvement of c...
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